Determination of Microgram Quantities of Inorganic Boron in Mammalian Specimens.
نویسندگان
چکیده
A method is presented for estimation of inorganic boron in mammalian tissues, blood, cerebrospinal fluid, and urine. The procedure is simple and rapid, qualifying it particularly to meet needs in clinical chemistry. The mammalian tissues are first enzymatically digested and extracted; then the deproteinized extract is allowed to react with quinalizarin in concentrated sulfuric acid. The intensity of the resultant color is read spectrophotometrically at 620 m . The boron concentration in the tissue analyzed is obtained from a standard reference graph for that tissue. The method is applicable to concentrations as low as I jtg. of boron per gram of mammalian tissue or fluid.
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عنوان ژورنال:
- Clinical chemistry
دوره 11 شماره
صفحات -
تاریخ انتشار 1965